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Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

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Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil1 Scope This International Standard specifies the crucial steps of a quantitative real time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups. It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging

without exceeding a specified temperature rise

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